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M94A0623.TXT
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1994-10-21
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Document 0623
DOCN M94A0623
TI Surface protein analysis of a non-syncytium inducing HIV-1 isolate.
DT 9412
AU Fryer KM; Deacon NJ; Land SA; McPhee DA; Macfarlane Burnet Centre for
Medical Research, Fairfield, Vic.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:72 (abstract no. SB3).
Unique Identifier : AIDSLINE ASHM5/94349032
AB An Australian HIV-1 isolate that is non-syncytium inducing (NSI), and
has reduced cytopathic effect on peripheral blood mononuclear cells
(PBMCs) has been characterised. The biochemical properties and DNA
sequence of this isolate (243925) were compared with a typical syncytium
inducing (SI) isolate 228200. Both isolates replicate well in PBMCs and
are neutralised by similar concentrations of sCD4 and OKT4a, but the NSI
isolate does not down-modulate CD4 from the surface of infected cells.
Phylogeny analysis based on envelope protein DNA sequence has mapped
both to clade B, with North American isolates. The nucleotide sequences
of the V3 loop of envelope of the two viruses correlate with published
syncytium and non-syncytium inducing virus sequences (Fouchier et al.,
1992). Western blot analysis has demonstrated greater heterogeneity in
the envelope protein on the surface of cells infected with 243925
compared with infection by the NSI isolate. The surface glycoprotein gp
120 was detected on the viral particles of the NSI isolate only,
suggesting that the protein is shed from the surface of the SI isolate.
These studies confirm the similarity of Australian HIV-1 isolates to
American isolates, and suggest a role for the stability of the envelope
protein in HIV-1 syncytium formation.
DE Australia Cell Line Cytopathogenic Effect, Viral/GENETICS DNA,
Viral/*GENETICS Gene Expression Regulation, Viral/PHYSIOLOGY Giant
Cells/*MICROBIOLOGY Human HIV Envelope Protein gp120/GENETICS
HIV-1/*GENETICS Monocytes/*MICROBIOLOGY T4 Lymphocytes/MICROBIOLOGY
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).